Search results for "Aboral ectoderm"

showing 2 items of 2 documents

cis-Regulatory sequences driving the expression of the Hbox12 homeobox-containing gene in the presumptive aboral ectoderm territory of the Paracentro…

2008

AbstractEmbryonic development is coordinated by networks of evolutionary conserved regulatory genes encoding transcription factors and components of cell signalling pathways. In the sea urchin embryo, a number of genes encoding transcription factors display territorial restricted expression. Among these, the zygotic Hbox12 homeobox gene is transiently transcribed in a limited number of cells of the animal-lateral half of the early Paracentrotus lividus embryo, whose descendants will constitute part of the ectoderm territory. To obtain insights on the regulation of Hbox12 expression, we have explored the cis-regulatory apparatus of the gene. In this paper, we show that the intergenic region …

Chromatin ImmunoPrecipitationDNA ComplementaryEmbryo Nonmammaliananimal structuresGreen Fluorescent ProteinsMolecular Sequence DataSettore BIO/11 - Biologia MolecolareEctodermHomeodomainMybBiologyOtxEctoderm specificationHomeobox cis-regulatory elements GFP sea urchinEctodermmedicineAnimalsRegulatory Elements TranscriptionalAboral ectodermSea urchin embryoMolecular BiologyGene transferDNA PrimersRegulator geneCis-regulatory moduleHomeodomain ProteinsGeneticsBase SequenceEmbryogenesisGene Expression Regulation DevelopmentalCell Biologycis-Regulatory moduleGastrulationmedicine.anatomical_structureMutagenesisRegulatory sequenceSea Urchinsembryonic structuresSoxHomeoboxSequence AlignmentDevelopmental BiologyDevelopmental Biology
researchProduct

A compact cis-regulatory module defines the exclusive expression of the Hbox12 homeobox-containing gene in the presumptive aboral ectoderm

2009

In the sea urchin embryo, a number of genes encoding transcription factors display territorial restricted expression. Among these, the zygotic Hbox12 homeobox gene is transiently transcribed in a limited number of cells of the animal-lateral half of the early Paracentrotus lividus embryo, whose descendants will constitute part of the ectoderm territory. We have explored the cis-regulatory apparatus of the gene and found that the intergenic region of the tandem Hbox12 repeats drives GFP expression in the presumptive aboral ectoderm and that a 234 bp fragment, defined Aboral Ectoderm Module, accounts for the restricted expression of the transgene. Within this module, a consensus sequence for …

Settore BIO/11 - Biologia Molecolaresea urchin embryo homeobox microinjection aboral ectoderm cis-regulatory elements
researchProduct